@article{202931,
  keywords = {immunoaffinity purification-mass spectrometry, protein interactions, label-free quantification, metabolic labeling, vesicular trafficking, SNARE, Arp2/3, AMPA receptors, LuTHy, synaptic biology, D. melanogaster},
  author = {Todd Greco and Christopher Secker and Eduardo Silva Ramos and Joel Federspiel and Jeh-Ping Liu and Alma Perez and Ismael Al-Ramahi and Jeffrey Cantle and Jeffrey Carroll and Juan Botas and Scott Zeitlin and Erich Wanker and Ileana Cristea},
  title = {Dynamics of huntingtin protein interactions in the striatum identifies candidate modifiers of Huntington disease},
  abstract = { Huntington disease (HD) is a monogenic neurodegenerative disorder with one causative gene, huntingtin (HTT). Yet, HD pathobiology is multifactorial, suggesting that cellular factors influence disease progression. Here, we define HTT protein-protein interactions (PPIs) perturbed by the mutant protein with expanded polyglutamine in the mouse striatum, a brain region with selective HD vulnerability. Using metabolically labeled tissues and immunoaffinity purification-mass spectrometry, we establish that polyglutamine-dependent modulation of HTT PPI abundances and relative stability starts at an early stage of pathogenesis in a Q140 HD mouse model. We identify direct and indirect PPIs that are also genetic disease modifiers using in-cell two-hybrid and behavioral assays in HD human cell and Drosophila models, respectively. Validated, disease-relevant mHTT-dependent interactions encompass mediators of synaptic neurotransmission (SNAREs and glutamate receptors) and lysosomal acidification (V-ATPase). Our study provides a resource for understanding mHTT-dependent dysfunction in cortico-striatal cellular networks, partly through impaired synaptic communication and endosomal-lysosomal system. A record of this paper{\textquoteright}s Transparent Peer Review process is included in the supplemental information. },
  year = {2022},
  journal = {Cell Syst.},
  volume = {13},
  pages = {304{\textendash}320.e5},
  publisher = {Elsevier BV},
  language = {eng},
}