@article{132411,
  keywords = {Animals, Binding Sites, Transcription Factors, Mice, Mice, Inbred C57BL, Cell Cycle Proteins, Histones, Embryonic Stem Cells, Genome, Telomere, Histone Chaperones, Transcription Initiation Site},
  author = {Aaron Goldberg and Laura Banaszynski and Kyung-Min Noh and Peter Lewis and Simon Elsaesser and Sonja Stadler and Scott Dewell and Martin Law and Xingyi Guo and Xuan Li and Duancheng Wen and Ariane Chapgier and Russell DeKelver and Jeffrey Miller and Ya-Li Lee and Elizabeth Boydston and Michael Holmes and Philip Gregory and John Greally and Shahin Rafii and Chingwen Yang and Peter Scambler and David Garrick and Richard Gibbons and Douglas Higgs and Ileana Cristea and Fyodor Urnov and Deyou Zheng and David Allis},
  title = {Distinct factors control histone variant H3.3 localization at specific genomic regions},
  abstract = {<p>The incorporation of histone H3 variants has been implicated in the epigenetic memory of cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we report genome-wide profiles of H3 variants in mammalian embryonic stem cells and neuronal precursor cells. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. The H3.3 chaperone Hira is required for H3.3 enrichment at active and repressed genes. Strikingly, Hira is not essential for localization of H3.3 at telomeres and many transcription factor binding sites. Immunoaffinity purification and mass spectrometry reveal that the proteins Atrx and Daxx associate with H3.3 in a Hira-independent manner. Atrx is required for Hira-independent localization of H3.3 at telomeres and for the repression of telomeric RNA. Our data demonstrate that multiple and distinct factors are responsible for H3.3 localization at specific genomic locations in mammalian cells.</p>
},
  year = {2010},
  journal = {Cell},
  volume = {140},
  pages = {678-91},
  month = {03/2010},
  issn = {1097-4172},
  doi = {10.1016/j.cell.2010.01.003},
  language = {eng},
}