@article{131976,
  keywords = {Spectrometry, Mass, Electrospray Ionization, Humans, Membrane Transport Proteins, Down-Regulation, Biomarkers, Female, Male, Aging, Proteome, Protein Interaction Maps, Tandem Mass Spectrometry, Alzheimer Disease, Aged, Middle Aged, Mitochondria, Retina, Up-Regulation, Blood Coagulation, Cholesterol, Eye Proteins, Complement Activation, Complement System Proteins, Electron Transport, Glaucoma, Neurodegenerative Diseases, Quality Control, Vitreous Body},
  author = {Mehdi Mirzaei and Veer Gupta and Joel Chick and Todd Greco and Yunqi Wu and Nitin Chitranshi and Roshana Vander Wall and Eugene Hone and Liting Deng and Yogita Dheer and Mojdeh Abbasi and Mahdie Rezaeian and Nady Braidy and Yuyi You and Ghasem Hosseini Salekdeh and Paul Haynes and Mark Molloy and Ralph Martins and Ileana Cristea and Steven Gygi and Stuart Graham and Vivek Gupta},
  title = {Age-related neurodegenerative disease associated pathways identified in retinal and vitreous proteome from human glaucoma eyes},
  abstract = {<p>ABSTARCT: Glaucoma is a chronic disease that shares many similarities with other neurodegenerative disorders of the central nervous system. This study was designed to evaluate the association between glaucoma and other neurodegenerative disorders by investigating glaucoma-associated protein changes in the retina and vitreous humour. The multiplexed Tandem Mass Tag based proteomics (TMT-MS3) was carried out on retinal tissue and vitreous humour fluid collected from glaucoma patients and age-matched controls followed by functional pathway and protein network interaction analysis. About 5000 proteins were quantified from retinal tissue and vitreous fluid of glaucoma and control eyes. Of the differentially regulated proteins, 122 were found linked with pathophysiology of Alzheimer{\textquoteright}s disease (AD). Pathway analyses of differentially regulated proteins indicate defects in mitochondrial oxidative phosphorylation machinery. The classical complement pathway associated proteins were activated in the glaucoma samples suggesting an innate inflammatory response. The majority of common differentially regulated proteins in both tissues were members of functional protein networks associated brain changes in AD and other chronic degenerative conditions. Identification of previously reported and novel pathways in glaucoma that overlap with other CNS neurodegenerative disorders promises to provide renewed understanding of the aetiology and pathogenesis of age related neurodegenerative diseases.</p>
},
  year = {2017},
  journal = {Sci Rep},
  volume = {7},
  pages = {12685},
  month = {10/2017},
  issn = {2045-2322},
  doi = {10.1038/s41598-017-12858-7},
  language = {eng},
}