@article{131951,
  keywords = {Humans, Gene Expression Regulation, Promoter Regions, Genetic, Transcription, Genetic, RNA, Messenger, Protein Binding, Mass Spectrometry, Staining and Labeling, Protein Transport, RNA Transport, RNA-Binding Proteins, Poly(A)-Binding Proteins, RNA Polymerase II, HEK293 Cells, RNA Stability, TATA-Box Binding Protein, Microtubule-Associated Proteins, Exoribonucleases},
  author = {Sarah Gilbertson and Joel Federspiel and Ella Hartenian and Ileana Cristea and Britt Glaunsinger},
  title = {Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription},
  abstract = {<p>Alterations in global mRNA decay broadly impact multiple stages of gene expression, although signals that connect these processes are incompletely defined. Here, we used tandem mass tag labeling coupled with mass spectrometry to reveal that changing the mRNA decay landscape, as frequently occurs during viral infection, results in subcellular redistribution of RNA binding proteins (RBPs) in human cells. Accelerating Xrn1-dependent mRNA decay through expression of a gammaherpesviral endonuclease drove nuclear translocation of many RBPs, including poly(A) tail-associated proteins. Conversely, cells lacking Xrn1 exhibited changes in the localization or abundance of numerous factors linked to mRNA turnover. Using these data, we uncovered a new role for relocalized cytoplasmic poly(A) binding protein in repressing recruitment of TATA binding protein and RNA polymerase II to promoters. Collectively, our results show that changes in cytoplasmic mRNA decay can directly impact protein localization, providing a mechanism to connect seemingly distal stages of gene expression.</p>
},
  year = {2018},
  journal = {Elife},
  volume = {7},
  month = {10/2018},
  issn = {2050-084X},
  doi = {10.7554/eLife.37663},
  language = {eng},
}